Product Support

Click Chemistry Protocols

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Cell Lysate Labeling

A general protocol for labeling cell lysate through a copper-catalyzed click reaction with any azide or alkyne click detection reagent.

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Cell Lysate Capture on Agarose Beads

A protocol for copper-catalyzed capturing of azide or alkyne labeled proteins on agarose beads from cell lysate.

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Copper-Free Cell Labeling

A protocol for a copper-free click reaction suitable for live imaging of azide-labeled cells and azide-labeled animals.

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Copper-Catalyzed Cell Labeling

A protocol for a copper-catalyzed click reaction suitable for live or fixed cell imaging and flow cytometry.

EdU Proliferation Assays

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EdU Proliferation Assay for Imaging

A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for imaging.

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EdU Plus Proliferation Assay for Imaging

A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for imaging use. The “Plus” version utilizes a more efficient click reaction using a new generation of copper chelating AZDye azides.

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EdU Proliferation Assay for Flow Cytometry

A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for analysis by flow cytometry.

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EdU Plus Proliferation Assay for Flow Cytometry

A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for analysis by flow cytometry. The “Plus” version utilizes a more efficient click reaction using a new generation of copper chelating AZDye azides.

Nancent Protein Sythethis Assays

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AHA/HPG

A protocol for nascent protein synthesis analysis by microscopy using clickable analogs of natural amino acids.

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OPP

A protocol for nascent protein synthesis analysis by microscopy using a clickable analog of puromycin.

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Glycoproteins labeling

A protocol for copper-catalyzed capturing of glycoproteins on agarose beads from cell lysate for analysis by mass spectrometry.

Protein Labeling Calculator

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Protein Labeling Calculator

Simple to use calculator that allows for determining amount of labeling reagent and organic co-solvent required for modification of proteins or any other biomolecules.