Click Chemistry Protocols
A general protocol for labeling cell lysate through copper-catalyzed click reaction with any azide or alkyne click detection reagent.
A protocol for imaging alkyne labeled DNA with fluorescent azides.
A protocol for imaging alkyne labeled DNA for flow cytometry.
A general protocol for S-phase labeling of the dividing cells with EdU.
Metabolic Labeling
A general protocol for S-phase labeling of the dividing cells with EdU.
L-Azidohomoalanine (AHA), L-Homopropargylglycine (HPG) Labeling
A protocol for preparation of AHA or HPG labeled cell lysates and conditioned media.
Metabolic Labeling of Glycoproteins
A protocol details the preparation of cell lysates metabolically labeled with azido-or alkynyl-sugar.
Protein Labeling Calculator
Simple to use calculator that allows for determining amount of labeling reagent and organic co-solvent required for modification of proteins or any other biomolecules.