Product Support
Click Chemistry Protocols
Cell Lysate Labeling
A general protocol for labeling cell lysate through a copper-catalyzed click reaction with any azide or alkyne click detection reagent.
Cell Lysate Capture on Agarose Beads
A protocol for copper-catalyzed capturing of azide or alkyne labeled proteins on agarose beads from cell lysate.
Copper-Free Cell Labeling
A protocol for a copper-free click reaction suitable for live imaging of azide-labeled cells and azide-labeled animals.
Copper-Catalyzed Cell Labeling
A protocol for a copper-catalyzed click reaction suitable for live or fixed cell imaging and flow cytometry.
EdU Proliferation Assays
EdU Proliferation Assay for Imaging
A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for imaging.
EdU Plus Proliferation Assay for Imaging
A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for imaging use. The “Plus” version utilizes a more efficient click reaction using a new generation of copper chelating AZDye azides.
EdU Proliferation Assay for Flow Cytometry
A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for analysis by flow cytometry.
EdU Plus Proliferation Assay for Flow Cytometry
A protocol for labeling of the dividing cells with EdU followed by a copper-catalyzed click reaction for analysis by flow cytometry. The “Plus” version utilizes a more efficient click reaction using a new generation of copper chelating AZDye azides.
Nancent Protein Sythethis Assays
AHA/HPG
A protocol for nascent protein synthesis analysis by microscopy using clickable analogs of natural amino acids.
OPP
A protocol for nascent protein synthesis analysis by microscopy using a clickable analog of puromycin.
Glycoproteins labeling
A protocol for copper-catalyzed capturing of glycoproteins on agarose beads from cell lysate for analysis by mass spectrometry.
Protein Labeling Calculator
Protein Labeling Calculator
Simple to use calculator that allows for determining amount of labeling reagent and organic co-solvent required for modification of proteins or any other biomolecules.