THPTA (tris-hydroxypropyltriazolylmethylamine) is a water-soluble, very effective accelerating ligand for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC). In addition to greatly enhancing the rate of CuAAC THPTA minimizes perturbations to the physiological state of the cells or organisms probed and allows for effective bioconjugation with suppressed cell cytotoxicity by further lowering CuI loading in the catalyst formulation. Water-soluble THPTA click ligand further simplifies Click Chemistry by allowing the entire reaction to be run in water, affording biological compatibility for Click Reactions.
|Solubility||Water, DMSO, DMF, MeOH|
|Appearance||Off-white to grey solid|
|Shipping conditions||Ambient temperature|
1. Hong, V., et al. (2009). Analysis and Optimization of Copper-Catalyzed Azide–Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed., 48: 9879 –9883, http://dx.doi.org/10.1002/anie.200905087.
2. Hong, V., et al. (2010). Labeling Live Cells by Copper-Catalyzed Alkyne-Azide Click Chemistry Bioconjugate Chem.,. 21(10): 1912–1916, DOI: 10.1021/bc100272z.
|BTTAA||A newest generation, water-soluble accelerating ligand for CuAAC.|
|Click-&-Go Protein Reaction Buffer Kit||A generic version of Click-iT® Protein Reaction Buffer Kit.|
|Click-&-Go Cell Reaction Buffer Kit||A generic version of Click-iT® Cell Reaction Buffer Kit.|
|AF 488 (Alexa Fluor® 488) Azide||A water-soluble, bright, green-emitting dye used for imaging low abundance alkyne-containing targets.|
|AF 488 (Alexa Fluor® 488) Alkyne||An alkyne-activated, water-soluble green emitting dye used for imaging low abundance azide-containing targets via CuAAC.|