Tetrazine-PEG5-NHS Ester is an amine-reactive labeling reagent often used for modification of proteins, peptides, or amine-modified oligonucleotides with tetrazine moiety. Hydrogen substituted tetrazines demonstrate exceptionally fast kinetics, generally at least 10 fold faster compared to methyl substituted tetrazines. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG5) spacer arm.
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