Terazine-activated TAMRA dye reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
|Flow cytometry laser line||532 555 or 568 nm|
|Microscopy laser line||532 or 555 nm|
|Spectrally similar dyes||Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549|
|Shipping conditions||Ambient temperature|