5(6)-TAMRA NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1074-25
25 mg
$115.00
In stock
1074-100
100 mg
$195.00
In stock
1074-1000
1000 mg
$895.00
In stock
1074-5G
5 g
$2,750.00
In stock

    5(6)-TAMRA NHS Ester (5(6)-Carboxytetramethylrhodamine, TMR, TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

    Abs/Em Maxima
    553/575 nm
    Extinction Coefficient
    92,000
    Flow Cytometry Laser Line
    532 nm or 555 nm
    Microscopy Laser Line
    532 nm or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, CF® 555, DyLight® 549
    Molecular weight
    527.53
    CAS
    150408-83-6
    Solubility
    Water, DMSO, DMF
    Purity
    >90% (HPLC)
    Appearance
    Dark red amorphous solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    5(6)-TAMRA NHS Ester (5(6)-Carboxytetramethylrhodamine, TMR, TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

    TAMRA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

    TAMRA

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