TAMRA DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
A131-1
1 mg
$115.00
In stock
A131-5
5 mg
$329.00
In stock
A131-25
25 mg
$1,195.00
In stock

TAMRA DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines. In application where the presence of copper is a concern TAMRA DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Abs/Em Maxima
548/562 nm
Extinction Coefficient
92,000
Spectrally Similar Dyes
Alexa Fluor® 546, Atto™ 543, CF® 543 Dye
Molecular weight
936.09
CAS
N/A
Solubility
DMSO, DMF
Purity
>95% (HPLC)
Appearance
Dark red amorphous solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

TAMRA DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This copper-free variation of click reaction overcomes a major shortcoming of copper-catalyzed click reaction – the need for copper catalyst. Copper can damage fluorescent proteins, Quantum Dot nanocyrstals, certain enzymes, and photoproteins like RPE. The presence of copper is also problematic in staining the surface of live cells. In application where the presence of copper is a concern TAMRA DBCO is an ideal alternative to copper requiring fluorescent alkynes.

TAMRA DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

TAMRA

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