Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label TAMRA Biotin Azide probe can be readily incorporated into alkyne-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence.
Another very important advantage of dual label probes over regular biotin probes is built-in control. Each step of enrichment process can easily followed either by UV-Vis (550 nm) or by more sensitive fluorescence spectroscopy. After elution form streptavidin beads target proteins containing TAMRA label can be easily distinguished from non-specifically bound proteins and endogenously biotinlytated proteins.
This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.
|TAMRA-Azide-Desthiobiotin||An analog of TAMRA-Azide-Biotin trifunctional reagent that allows for easy elution from streptavidin.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|
|Click-&-Go™ Protein Reaction Buffer Kit||All-inclusive kit that provides everything required to perform CuAAC on azide or alkyne tagged proteins with the corresponding click detection reagent.|