QuántiLink Biotin-PEG12-NHS Ester
QuántĭLinkTM Biotin labeling reagents comprise a family of the most advances biotinylation reagents with built-in signal quantification capability. These reagents contain UV signature that enables direct nondestructive quantification of total incorporated biotin molecules by means of spectroscopic (A280/A350) measurement of modified proteins.
Hydrophilic PEG12 arms enhance water solubility, reduce the tendency of some proteins to aggregate/precipitate during biotinylation, and improve conjugate yield at moderately-to-higher biotin loads.
After biotinylation, the degree of biotinylation can be measured in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple, non-destructive UV-Vis scan you can quantify biotin incorporation and ensure reproducible production of consistent batches.
|Solubility||DMSO, DMF, THF, DCM|
|Appearance||Slightly yellow oil|
|Storage conditions||-20°C. Desiccate|
|Shipping conditions||Ambient temperature|
Protein Labeling Calculator
** Please fill all the required input **
Volume of DMSO required to make a stock solution (µL)
Volume of protein to be labeled (µL)
Volume of biotin stock solution to add (µL)
Percent DMSO in Reaction Mixture (%)
Caution - Volume of DSMO solvent added to protein must always be <5% total volume of reaction