Protein A/G Magnetic Beads
Catalog#
Unit Size
Price (USD)
Availability
Qty
1560-1
1 ml
$149.00
In stock
1560-5
5 ml
$520.00
In stock
Click Chemistry Tools high binding capacity protein A/G magnetic beads are ideally suitable for antibody purification and immunoprecipitation both manually and automatically. These beads utilize Protein A/G which is a genetically engineered protein that combines IgG binding domains of both Protein A and Protein G allowing to capture antibodies from different species.
Enrichment target
Antibodies (IgG)
Capacity
55-85 µg IgG per mg of beads
Support
Highly uniformed supermagnetic particles
Coating
Highly crosslinked, proprietary (non-styrene) polymer matrix
Mean bead diameter
0.8 µm
Formulation
10 mg/mL suspension in PBS supplemented with 0.1% BSA, 0.05% Tween® 20
Preservative
0.05% sodium azide
Appearance
Brown suspension
Storage
2-8C
Shipping conditions
Blue ice
- High capacity— reduces amount of beads required for efficient antibody binding, resulting in low background, better signal and lower net cost
- Low non-specific binding— albumin-binding site of the Protein G has been eliminated resulting in cleaner purification products
- Universal— no need to use Protein A and Protein G beads separately for different IgG. Protein A/G binds all IgG from all commonly used species
- Compatibility— suitable for both manual and automated applications
Assay consistency— no beads loss compared to classical resin-based IP methods
Applications:
• Immunoprecipitation (IP) and Co-IP
• Antibody purification
- Do not centrifuge, freeze or dry
- Do not vortex
• Include 0.05% non-ionic (e.g., Tween™-20) or zwitterionic (e.g., CHAPS) detergent in the binding buffer for optimal antibody binding
