Protein A/G Magnetic Beads

1560-1

1 ml

$149.00

In stock

1560-5

5 ml

$520.00

In stock

Click Chemistry Tools high binding capacity protein A/G magnetic beads are ideally suitable for antibody purification and immunoprecipitation both manually and automatically. These beads utilize Protein A/G which is a genetically engineered protein that combines IgG binding domains of both Protein A and Protein G allowing to capture antibodies from different species.

Enrichment target

Antibodies (IgG)

Capacity

55-85 µg IgG per mg of beads

Support

Highly uniformed supermagnetic particles

Coating

Highly crosslinked, proprietary (non-styrene) polymer matrix

Mean bead diameter

0.8 µm

Formulation

10 mg/mL suspension in PBS supplemented with 0.1% BSA, 0.05% Tween® 20

Preservative

0.05% sodium azide

Appearance

Brown suspension

Storage

2-8C

Shipping conditions

Blue ice

Magnetic Beads structure

High capacity— reduces amount of beads required for efficient antibody binding, resulting in low background, better signal and lower net cost
Low non-specific binding— albumin-binding site of the Protein G has been eliminated resulting in cleaner purification products
Universal— no need to use Protein A and Protein G beads separately for different IgG. Protein A/G binds all IgG from all commonly used species
Compatibility— suitable for both manual and automated applications

Assay consistency— no beads loss compared to classical resin-based IP methods

Applications:
• Immunoprecipitation (IP) and Co-IP
• Antibody purification

Do not centrifuge, freeze or dry
Do not vortex

• Include 0.05% non-ionic (e.g., Tween™-20) or zwitterionic (e.g., CHAPS) detergent in the binding buffer for optimal antibody binding