Protein A/G Magnetic Beads

Catalog#
Unit Size
Price (USD)
Availability
Qty
1560-1
1 ml
$149.00
In stock
1560-5
5 ml
$520.00
In stock

    Click Chemistry Tools high binding capacity protein A/G magnetic beads are ideally suitable for antibody purification and immunoprecipitation both manually and automatically. These beads utilize Protein A/G which is a genetically engineered protein that combines IgG binding domains of both Protein A and Protein G allowing to capture antibodies from different species.

    Enrichment target
    Antibodies (IgG)
    Capacity
    55-85 µg IgG per mg of beads
    Support
    Highly uniformed supermagnetic particles
    Coating
    Highly crosslinked, proprietary (non-styrene) polymer matrix
    Mean bead diameter
    0.8 µm
    Formulation
    10 mg/mL suspension in PBS supplemented with 0.1% BSA, 0.05% Tween® 20
    Preservative
    0.05% sodium azide
    Appearance
    Brown suspension
    Storage
    2-8C
    Shipping conditions
    Blue ice

    Magnetic Beads structure

    • High capacity— reduces amount of beads required for efficient antibody binding, resulting in low background, better signal and lower net cost
    • Low non-specific binding— albumin-binding site of the Protein G has been eliminated resulting in cleaner purification products
    • Universal— no need to use Protein A and Protein G beads separately for different IgG. Protein A/G binds all IgG from all commonly used species
    • Compatibility— suitable for both manual and automated applications

    Assay consistency— no beads loss compared to classical resin-based IP methods

    Applications:
    • Immunoprecipitation (IP) and Co-IP
    • Antibody purification

    • Do not centrifuge, freeze or dry
    • Do not vortex

    • Include 0.05% non-ionic (e.g., Tween™-20) or zwitterionic (e.g., CHAPS) detergent in the binding buffer for optimal antibody binding