6-Azido-2,4-diacetamido-2,4,6-trideoxy-D-mannose (pLeg-Azide) is an analogue of the natural 2,4-diacetamido-2,4-dideoxy-D-mannose (pLeg) that contains a very small modification, specifically an azido moiety. This molecule incorporated by cultured cells (Legionella pneumophila, for example) and incorporated into glycan (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or “click” reaction between an azide and an alkyne or cyclooctyne. The azido modified glycan is detected with either a fluorescent alkyne or a biotin alkyne. Azide and alkyne groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency.
|Solubility||DMSO, DMF, Water, MeOH|
|Appearance||White lyophilized powder|
|Shipping conditions||Ambient temperature|
1. Dumont, A., et al. (2014). Identification of Living Legionella pneumophila Using Species-Specific Metabolic Lipopolysaccharide Labeling Angew. Chem., Int. Ed., 53: 1275–1278.