PC Biotin-NHS ester

Catalog # Pkg Size Price(USD) Quantity
1225-1 4 x 1 mg $149.00
1225-10 10 mg $149.00
1225-25 25 mg $245.00
1225-100 100 mg $695.00

PC Biotin-NHS Ester as a unique amine reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

 

Protein Labeling Calculator

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  • Volume of DMSO required to make a stock solution (µL)

  • Volume of protein to be labeled (µL)

  • Volume of biotin stock solution to add (µL)

  • Percent DMSO in Reaction Mixture (%)

Caution - Volume of DSMO solvent added to protein must always be <5% total volume of reaction