PB Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1413-1
1 mg
$179.00
In stock
1413-5
5 mg
$495.00
In stock
1413-25
25 mg
$1,795.00
In stock

    PB Picolyl Azide (Pacific Blue® Picolyl Azide equivalent) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Abs/Em Maxima
    416/451 nm
    Extinction Coefficient
    30,000
    Spectrally Similar Dyes
    Alexa Fluor® 405, CF® 405, Pacific Blue®, DyLight® 405
    Molecular weight
    444.35
    CAS
    N/A
    Solubility
    DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Yellow amorphous solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    PB Picolyl Azide (Pacific Blue® Picolyl Azide equivalent) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    PB (Pacific Blue® equivalent) is a moderately photostable, blue-fluorescent probe optimally excited by the 405 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

    Pacific Blue® is a registered trademark of Thermo Fisher Scientific.

    pacific_blue

    1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
    2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
    3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
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