O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.
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An acid-cleavable biotin probe that allows for elution of the captured biomolecules under mild conditions (5%-10% formic acid).
An assay kit that enables sensitive, and non-radioactive detection of nascent protein synthesis in complete, methionine-containing media using green-fluorescent AZDye 488.
A novel and robust nascent protein synthesis assay based on an alkyne containing and cell-permeable analog of puromycin, O-Propargyl-puromycin (OPP) that stops translation by forming covalent conjugates with nascent polypeptide chains.