O-propargyl-puromycine (OPP)
O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.
- Morral, C., et al. (2020). Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell., 26 (6), 845-861.e12. [PubMed]
- Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry, 10, 1093. [Oxford Academic]
- Lui, J., et al. (2012). Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. PNAS, 109, 413-8. [PubMed]
- Forester, C.M., et al. (2018). Revealing nascent proteomics in signaling pathways and cell differentiation.PNAS, 115, 2353-8. [PubMed]
- Tong, M., et al. (2020). Effective Method for Accurate and Sensitive Quantitation of Rapid Changes of Newly Synthesized Proteins. Anal. Chem., 92(14), 10048-57. [PubMed]
Leach, R. W., et al. (2021). Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Nat Chem Biol., 17 (11), 1178-1187. [PubMed]
Excitation maximum: 494 nm
Emission maximum: 517 nm
Excitation maximum: 648 nm
Emission maximum: 671 nm
An azide-activated biotinylation reagent.
A novel and robust nascent protein synthesis assay based on an alkyne containing and cell-permeable analog of puromycin, O-Propargyl-puromycin (OPP) that stops translation by forming covalent conjugates with nascent polypeptide chains.
An acid-cleavable biotin probe that allows for elution of the captured biomolecules under mild conditions (5%-10% formic acid).
An assay kit that enables sensitive, and non-radioactive detection of nascent protein synthesis in complete, methionine-containing media using green-fluorescent AZDye 488.