O-propargyl-puromycine (OPP)

Catalog#
Unit Size
Price (USD)
Availability
Qty
1407-5
5 mg
$345.00
In stock
1407-25
25 mg
$1,295.00
In stock
1407-100
100 mg
$2,820.00
In stock

    O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.

    Molecular weight
    495.54
    Chemical composition
    C24H29N7O5
    CAS
    1416561-90-4
    Solubility
    DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Off-white to slightly grey crystalline
    Storage conditions
    -20°C.
    Shipping conditions
    Ambient temperature

    1. Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry, 10, 1093. [Oxford Academic]
    2. Lui, J., et al. (2012). Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. PNAS, 109, 413-8. [PubMed]
    3. Forester, C.M., et al. (2018). Revealing nascent proteomics in signaling pathways and cell differentiation.PNAS, 115, 2353-8. [PubMed]
    4. Tong, M., et al. (2020). Effective Method for Accurate and Sensitive Quantitation of Rapid Changes of Newly Synthesized Proteins. Anal. Chem., 92(14), 10048-57. [PubMed]
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