O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.
An acid-cleavable biotin probe that allows for elution of the captured biomolecules under mild conditions (5%-10% formic acid).
Excitation maximum: 648 nm
Emission maximum: 671 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm
An imaging kit for click chemistry detection of alkyne-tagged proteins with the green-fluorescent AFDye 488.
An azide-activated biotinylation reagent.