OG 488 Alkyne
OG 488 Alkyne, (also known as Oregon Green® 488 Alkyne or 2′,7′-Difluorocarboxyfluorescein Alkyne, 6-isomer) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. OG 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
Difluorocarboxyfluorescein is more photostability and ionizes at a lower pH (pKa 4.8) than fluorescein (pKa 6.5), which makes it fluorescent even at slightly acidic pH where conventional fluorescein is virtually non-fluorescent. The absorption and emission of OG 488 dye are virtually identical to widely used fluorescein dye.
OG 488 Alkyne, (also known as Oregon Green® 488 Alkyne or 2′,7′-Difluorocarboxyfluorescein Alkyne, 6-isomer) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. OG 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
The fluorinated carboxyfluoresceins have higher photostability and ionize at a lower pH (pKa 4.8) than fluorescein (pKa 6.5), which makes them superior fluorescent dyes for use as reporter molecules in biological systems. The absorption and emission of OG 488 dye are virtually identical to widely used fluorescein dyes.
The combination of higher photostability, lower pKa, and excitation ideally suited to the 488 nm laser line makes OG 488 dye an ideal replacement for the widely used fluorescein dyes.
Oregon Green® is a registered trademark of Thermo Fisher Scientific.
A click chemistry reaction buffer kit optimized for cell lysates labeling.
Excitation maximum: 494 nm
Emission maximum: 517 nm
A newest generation, water-soluble accelerating ligand for CuAAC.