OG 488 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1397-1
1 mg
$95.00
In stock
1397-5
5 mg
$295.00
In stock
1397-25
25 mg
$895.00
In stock
1397-100
100 mg
$1,995.00
In stock

    OG 488 Alkyne, (also known as Oregon Green® 488 Alkyne or 2′,7′-Difluorocarboxyfluorescein Alkyne, 6-isomer) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. OG 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

    Difluorocarboxyfluorescein is more photostability and ionizes at a lower pH (pKa 4.8) than fluorescein (pKa 6.5), which makes it fluorescent even at slightly acidic pH where conventional fluorescein is virtually non-fluorescent. The absorption and emission of OG 488 dye are virtually identical to widely used fluorescein dye.

    Abs/Em Maxima
    496/524 nm
    Extinction Coefficient
    84,000
    Flow Cytometry Laser Line
    488 nm
    Microscopy Laser Line
    488 nm
    Spectrally Similar Dyes
    Fluorescein, Alexa Fluor® 488, Atto™ 488, CF® 488A dyes, DyLight® 488
    Molecular weight
    449.37
    CAS
    N/A
    Solubility
    DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Orange to red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    OG 488 Alkyne, (also known as Oregon Green® 488 Alkyne or 2′,7′-Difluorocarboxyfluorescein Alkyne, 6-isomer) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. OG 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

    The fluorinated carboxyfluoresceins have higher photostability and ionize at a lower pH (pKa 4.8) than fluorescein (pKa 6.5), which makes them superior fluorescent dyes for use as reporter molecules in biological systems. The absorption and emission of OG 488 dye are virtually identical to widely used fluorescein dyes.

    The combination of higher photostability, lower pKa, and excitation ideally suited to the 488 nm laser line makes OG 488 dye an ideal replacement for the widely used fluorescein dyes.

    Oregon Green® is a registered trademark of Thermo Fisher Scientific.

    AZDye488

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