Mono-sulfone is a thiol-specific labeling reagent that undergo alkylation to generate a thio-ether bond between the protein and mono-sulfone activated reagent. Once mono-sulfone reagent is conjugated to a protein or peptide, exchange reactions leading to deconjugation can be easily avoided by treating the conjugate with a mild reducing agent in a way analogous to the process used for conjugation reactions involving reductive amination.
- Badescu, G., et al. (2014). A New Reagent for Stable Thiol-Specific Conjugation. Bioconjug. Chem., 25(3), 460-9. [PubMed]