MB 594 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1181-1
1 mg
$149.00
In stock
1181-5
5 mg
$435.00
In stock
1181-25
25 mg
$1,495.00
In stock

MB™ 594 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

Abs/Em Maxima
601/623 nm
Extinction Coefficient
110,000
Flow Cytometry Laser Line
532 or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes
Alexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594
Molecular weight
1050.19
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

MB™ 594 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

MB™ 594 is a bright, red fluorescent probe with absorption and emission maxima similar to Texas Red dye or Alexa Fluor® 594. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 594 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 594 can be used as a replacement for Alexa Fluor® 594, DyLight® 594, and Texas Red.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific.

MB594

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

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