MB 594 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1184-1
1 mg
$149.00
In stock
1184-5
5 mg
$455.00
In stock
1184-25
25 mg
$1,395.00
In stock

    MB™ 594 is a bright, red fluorescent probe with absorption and emission maxima similar to Texas Red dye or Alexa Fluor® 594. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 594 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 594 can be used as a high performance replacement for Alexa Fluor® 594, DyLight® 594, and Texas Red.

    Abs/Em Maxima
    601/623 nm
    Extinction Coefficient
    110,000
    Flow Cytometry Laser Line
    532 or 594 nm
    Microscopy Laser Line
    594 nm
    Spectrally Similar Dyes
    Alexa Fluor® 594, Atto™ 594, CF® 594 Dye, DyLight® 594
    Molecular weight
    1102.26
    CAS
    N/A
    Solubility
    Water, DMSO, DMF, MeOH
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    MB™ 594 is a bright, red fluorescent probe with absorption and emission maxima similar to Texas Red dye or Alexa Fluor 594®. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 594 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 594 can be used as a replacement for Alexa Fluor 594, DyLight 594, and Texas Red.

    MB™ 594 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 594 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    MB™ 594 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

    DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

    MB594

    Related Products
    AFDye 488 DBCO

    Excitation maximum: 494 nm
    Emission maximum: 517 nm

    AFDye 647 DBCO

    Excitation maximum: 648 nm
    Emission maximum: 671 nm