MB 594 DBCO

MB™ 594 is a bright, red fluorescent probe with absorption and emission maxima similar to Texas Red dye or Alexa Fluor 594®. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 594 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 594 can be used as a replacement for Alexa Fluor 594, DyLight 594, and Texas Red.

MB™ 594 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 594 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

MB™ 594 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.