MB 543 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1174-1
1 mg
$149.00
In stock
1174-5
5 mg
$395.00
In stock
1174-25
25 mg
$1,295.00
In stock

    MB™ 543 DBCO is an orange colored fluorescent probe with absorption and emission maxima at 544 and 560 nm, respectively. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 543 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 543 fluorescent dyes can be used in place of TAMRA, Alexa Fluor 546®, DyLight 546®, CF™543 and other spectrally similar dyes.

    Abs/Em Maxima
    543/563 nm
    Extinction Coefficient
    105,000
    Flow Cytometry Laser Line
    532 nm
    Microscopy Laser Line
    543 or 546 nm
    Spectrally Similar Dyes
    Alexa Fluor® 546, Atto™ 543, CF® 543
    Molecular weight
    1074.34
    CAS
    N/A
    Solubility
    Water, DMSO, DMF, MeOH
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    MB™ 543 DBCO is an orange colored fluorescent probe with absorption and emission maxima at 544 and 560 nm, respectively. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 543 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 543 fluorescent dyes can be used in place of TAMRA, Alexa Fluor 546®, DyLight 546®, CF™543 and spectrally similar dyes.

    MB™ 543 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 543 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    MB™ 543 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

    DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

    MB543

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