MB 543 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1174-1
1 mg
$149.00
In stock
1174-5
5 mg
$395.00
In stock
1174-25
25 mg
$1,295.00
In stock

MB™ 543 DBCO is an orange colored fluorescent probe with absorption and emission maxima at 544 and 560 nm, respectively. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 543 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 543 fluorescent dyes can be used in place of TAMRA, Alexa Fluor 546®, DyLight 546®, CF™543 and other spectrally similar dyes.

Abs/Em Maxima
543/563 nm
Extinction Coefficient
105,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 or 546 nm
Spectrally Similar Dyes
Alexa Fluor® 546, Atto™ 543, CF® 543
Molecular weight
1074.34
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

MB™ 543 DBCO is an orange colored fluorescent probe with absorption and emission maxima at 544 and 560 nm, respectively. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 543 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 543 fluorescent dyes can be used in place of TAMRA, Alexa Fluor 546®, DyLight 546®, CF™543 and spectrally similar dyes.

MB™ 543 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 543 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

MB™ 543 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

MB543

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