MB 488 Picolyl Azide
MB™ 488 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concentration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of MB™ 488 Picolyl Azide instead of conventional MB™ 488 Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
MB™ 488 Picolyl Azide is a bright, green fluorescent probe with excitation ideally suited for the 488 nm laser line. MB™ 488 is bright, exceedingly photostable and pH-insensitive fluorescent dye that can be used in place of Fluorescein, Alexa Fluor 488®, DyLight 488® and other dyes excitable at a wavelength of 488 nm.
Learn more about picolyl azide reagents.
|Abs/Em Maxima||495/515 nm|
|Flow cytometry laser line||488 nm|
|Microscopy laser line||488 nm|
|Spectrally similar dyes||FAM, Alexa Fluor® 488, Atto™ 488, CF™ 488A dyes, DyLight™488,|
|Molecular weight||ca. 850|
|Solubility||DMSO, DMF, MeOH|
|Appearance||Red to orange solid|
|Shipping conditions||Ambient temperature|
1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem., 25(4): 698-706.
2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,. 51(24): 5852–5856.
3. Gaebler, A., et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res.. DOI: 10.1194/jlr.D070565.
|EZ-Click Plus MB 488 Labeling Kit||A labeling kit that provides increased sensitivity and faster reaction times compared to labeling kits based on standard azides.|
|Click Chemistry Protein Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|