L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
|Molecular weight||180.59 (HCl salt)|
|Chemical composition||C4H9ClN4O2 (HCl salt)|
|Solubility||Water, DMSO, DMF|
|Purity||>95% (H NMR)|
|Shipping conditions||Ambient temperature|