L-Azidohomoalanine (AHA)

Catalog#
Unit Size
Price (USD)
Availability
Qty
1066-25
25 mg
$95.00
In stock
1066-100
100 mg
$195.00
In stock
1066-1000
1000 mg
$495.00
In stock
1066-5G
5 g
$1,395.00
In stock

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Molecular weight
180.59 (HCl salt)
Chemical composition
C4H9ClN4O2 (HCl salt)
CAS
942518-29-8
Solubility
Water, DMSO, DMF
Purity
>95% (H NMR)
Appearance
White crystalline
Storage conditions
-20°C.
Shipping conditions
Ambient temperature

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Biotin Azide

An azide-activated biotinylation reagent.