L-Azidohomoalanine (AHA)

Unit Size
Price (USD)
25 mg
In stock
100 mg
In stock
1000 mg
In stock
5 g
In stock

    L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

    Molecular weight
    180.59 (HCl salt)
    Chemical composition
    C4H9ClN4O2 (HCl salt)
    Water, DMSO, DMF
    >95% (H NMR)
    White crystalline
    Storage conditions
    Shipping conditions
    Ambient temperature

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