L-Azidohomoalanine (AHA)

Catalog#
Unit Size
Price (USD)
Availability
Qty
1066-25
25 mg
$115.00
In stock
1066-100
100 mg
$235.00
In stock
1066-1000
1000 mg
$595.00
In stock
1066-5G
5 g
$1,535.00
In stock

    L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

    Molecular weight
    180.59 (HCl salt)
    Chemical composition
    C4H9ClN4O2 (HCl salt)
    CAS
    942518-29-8
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (H NMR)
    Appearance
    White crystalline
    Storage conditions
    -20°C.
    Shipping conditions
    Ambient temperature

    1. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]
    2. Coates, H. W. et al. (2021). The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form. J Biol Chem., Online ahead of print. [PubMed]
    3. Smeekens, J. M., et al. (2020). Systematic quantification of the dynamics of newly synthesized proteins unveiling their degradation pathways in human cells. Chem Sci., 11 (13), 3557-3568. [PubMed]
    4. Tao, W.A., et al. (2019). Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics. Mass Spectrometry‐Based Chemical Proteomics. [PubMed]
    5. Dumont, A. A., et al. (2018). ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression. Sci Rep., 8 (1), 13605. [PubMed]
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