L-Azidohomoalanine

Catalog # Pkg Size Price(USD) Quantity
1066-25 25 mg $95.00
1066-100 100 mg $195.00
1066-1000 1000 mg $495.00
1066-5G 5 g $1,395.00

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.