Kdo-Azide

Catalog # Pkg Size Price(USD) Quantity
1241-10 10 mg $195.00
1241-25 25 mg $395.00
1241-100 100 mg $895.00

8-Azido-3,8-dideoxy-D-manno-octulosonic acid (Kdo-Azide) is an analogue of the natural 3-deoxy-D-manno-octulosonic acid (Kdo) that contains a very small modification, specifically an azido moiety. This molecule can be incorporated by cultured cells (Gram-negative Bacteria, for example) and incorporated into glycans (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or “click” reaction between an azide and an alkyne or cyclooctyne. The azido modified glycan can be labeled with either a fluorescent alkyne or a biotin alkyne. Azide and alkyne groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency.
 

 

1. Dumont, A., et al. (2012). Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core. Angew. Chem., Int. Ed., 51: 3143-3146.