8-Azido-3,8-dideoxy-D-manno-octulosonic acid (Kdo-Azide) is an analogue of the natural 3-deoxy-D-manno-octulosonic acid (Kdo) that contains a very small modification, specifically an azido moiety. This molecule can be incorporated by cultured cells (Gram-negative Bacteria, for example) and incorporated into glycans (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or “click” reaction between an azide and an alkyne or cyclooctyne. The azido modified glycan can be labeled with either a fluorescent alkyne or a biotin alkyne. Azide and alkyne groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency.
|Solubility||DMSO, DMF, Water|
|Appearance||White lyophilized powder|
|Shipping conditions||Ambient temperature|
1. Dumont, A., et al. (2012). Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core. Angew. Chem., Int. Ed., 51: 3143-3146.
|Cy5 DBCO||A water-soluble, azide-reactive near-IR probe.|
|TAMRA DBCO||An orange-to-red fluorescence-emitting probe with excitation/emission maximum of 554/567 nm.|
|WS DBCO-Biotin||A water-soluble, azide-reactive biotinylation reagent.|
|DBCO-PEG4-Biotin||An azide-reactive biotinylation reagent with a hydrophilic PEG4 spacer arm.|