IsoDTB (Isotopically labeled Desthoibiotin Azide) probes take advantage of the mass shift of stable heavy isotope labels (SHLs) to enable mass-independent chemical proteomics platform that helps to address unique challenges of the proteome characterization. In this approach a unique isotopic signature is embedded exclusively into the peptides and it serves as a computationally recognizable full-scan MS reporter. By using desthiobiotin, these probes circumvented the need to use cleavable linkers for peptide elution and thus simplifies the chemoproteomic protocol, while allowing quantification of the proteome. IsoTDB pack contains 2 mg of light (IsoDTB-L) and heave (IsoDTB) probe.
- Patric, R.A., et al. (2020).Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome .Angew. Chem. Int. Ed., 59: 2829-36. [PubMed]