Fluorescein Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1180-1
1 mg
$139.00
In stock
1180-5
5 mg
$295.00
In stock
1180-25
25 mg
$1,095.00
In stock

Fluorescein Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

Abs/Em Maxima
490/510 nm
Extinction Coefficient
76,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488
Molecular weight
577.53 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Fluorescein Picolyl Azide Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AFDye488

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

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