Disulfide Azide Agarose
Disulfide Azide Agarose is a 6% crosslinked agarose resin that is activated with azide groups through a cleavable disulfide linker. Once biomolecules are covalently captures to the resin, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins. Cleavage under reducing conditions, such as 2% 2-Mercaptoethanol, yields a highly enriched population of proteins. This product is adequate to work in batch or column purification (low pressure).
Batch or column purifications
5-20 µmol azide groups per mL resin
Spherical, 50-150 µm
Molecular weight left behind