Desthiobiotin NHS Ester
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
(a) Hirsch, J.D., at al. (2002). Anal. Biochem., 308: 343-357. (b) Hofmann, K., et al. (1982) Biochemistry, 21: 978-984. (c) Romovacek, H., et al. (1983) Biochemistry, 22: 904-909. (d) Finn, F.M., et al. (1984) Biochemistry, 23: 2554-2558. (e) Busse, S., et al. (2002) Biosensors and Bioelectronics, 17: 704-710. (f) Yoon, H.C., et al. Langmuir, 17 1234-1239.
|Shipping conditions||Ambient temperature|