Desthiobiotin NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1201-100
100 mg
$129.00
In stock
1201-500
500 mg
$495.00
In stock
1201-1000
1000 mg
$795.00
In stock

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Molecular weight
311.34
Chemical composition
C14H21N3O5
CAS
80750-24-9
Solubility
DMSO, DMF
Purity
>95%
Appearance
White solid
Storage conditions
-20°C
Shipping conditions
Ambient temperature