Desthiobiotin Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1107-5
5 mg
$89.00
In stock
1107-25
25 mg
$395.00
In stock
1107-100
100 mg
$1,195.00
In stock

    Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

    Molecular weight
    414.50
    Chemical composition
    C18H34N6O5
    CAS
    1306615-47-3
    Solubility
    DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Colorless to slightly yellow oil
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    1. David, L. L., et al. (2021). Chemical Proteomics Approach for Profiling the NAD Interactome. J Am Chem Soc., 143 (18), 6787-6791. [PubMed]