Desthiobiotin Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1107-5
5 mg
$95.00
In stock
1107-25
25 mg
$329.00
In stock
1107-100
100 mg
$1,095.00
In stock

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Molecular weight
414.50
Chemical composition
C18H34N6O5
CAS
1306615-47-3
Solubility
DMSO, DMF
Purity
>95% (HPLC)
Appearance
Colorless to slightly yellow oil
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature