Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Dde biotin probes eliminate a major limitation of the streptavidin-biotin affinity purification associated with extraordinary strength of the streptavidin-biotin interaction. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a Dde linker. Captured biomolecules can be efficiently released, typically >90%.
|Molecular weight left behind||100.07|
|Solubility||DMSO, DMF, THF, DCM, Chloroform|
|Shipping conditions||Ambient temperature|
1. Yang Y., et al. (2013). Cleavable Trifunctional Biotin Reagents for Protein Labeling, Capture, and Release. Chem. Commun., 48: 5366-5386.
2. Matthew E. G., et al. (2017). Comprehensive Mapping of O-GlcNAc Modification Sites Using a Chemically Cleavable Tag. Mol. Biosyst, 12(6): 1756–1759.
3. Gertsik N., et al. (2017). Mapping the Binding Site of BMS-708163 on y-Secretase with Cleavable Photoprobes. Cell Chemical Biology, 32: 3-8.
|Dde Biotin Picolyl Azide||Cleavable biotin probe with superior kinetics in copper-catalyzed click reactions.|
|Click-&-Go Protein Reaction Buffer Kit||A generic version of Click-iT® Protein Reaction Buffer Kit.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|