DBCO-Sulfo-NHS Ester
DBCO-Sulfo-NHS Ester is water-soluble reagent that enables simple and efficient labeling of antibodies, proteins and any other primary amine-containing macromolecules with DBCO moiety in 100% aqueous buffers. It reacts with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic pH to form covalent bonds.
DBCO-Sulfo-NHS Ester Ester is water-soluble reagent that enables simple and efficient labeling of antibodies, proteins and any other primary amine-containing macromolecules with DBCO moiety in 100% aqueous buffers. It reacts with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic pH to form covalent bonds. DBCO-Sulfo-NHS Ester is a reagent of choice for applications that cannot tolerate organic co-solvents or are complicated by their inclusion. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.
DBCO reagents, also known as ADIBO or DIBAC, are the most commonly used substrates for strain-promoted click chemistry reaction. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.
- Wiener, J., et al. (2020). Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics. Sci Rep., 10 (1), 1457. [PubMed]
An amine-reactive reagents with enhanced labeling efficiency.
A water-soluble labeling reagent with improved labeling efficiency.
A water-soluble activated ester for modification of primary amine-containing molecules with sulfo DBCO moiety.