Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
DMSO, DMF, DCM, THF, Chloroform
Colorless to slightly yellow oil
Dde Biotin DBCO
A hydrazine cleavable, azide-reactive biotin probe.
PC DBCO Biotin
An azide-reactive photoclevable biotin probe.