Desthiobiotin-PEG4-DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1108-10
10 mg
$179.00
In stock
1108-25
25 mg
$329.00
In stock
1108-100
100 mg
$1,095.00
In stock

    Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

    Molecular weight
    719.87
    Chemical composition
    C39H51N5O8
    CAS
    N/A
    Solubility
    DMSO, DMF, DCM, THF, Chloroform
    Purity
    >95% (HPLC)
    Appearance
    Colorless to slightly yellow oil
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

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