DBCO-mPEG, 5 kDa
DBCO-activated PEGylation reagents react with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern DBCO-activated PEGylation reagents is an ideal alternative to copper requiring fluorescent alkynes.
- Hunt, J. P., et al. (2022). Assessing site-specific PEGylation of TEM-1 β-lactamase with cell-free protein synthesis and coarse-grained simulation. J Biotechnol., 345:55-63, Epub. [PubMed]
- Wilding, K. M., et al. (2018). The Locational Impact of Site-Specific PEGylation: Streamlined Screening with Cell-Free Protein Expression and Coarse-Grain Simulation. ACS Synth Biol., 7 (2), 510-521. [PubMed]