Cy7 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1183-1
1 mg
$149.00
In stock
1183-5
5 mg
$395.00
In stock
1183-25
25 mg
$1,295.00
In stock

    Cy7 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Abs/Em Maxima
    753/775 nm
    Extinction Coefficient
    255,000
    Spectrally Similar Dyes
    Alexa Fluor® 750, RDye® 750, CF® 750 Dye, DyLight® 750
    Molecular weight
    1203.43
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Dark green solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy7 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    Cy7 Picolyl Azide is a bright and photostable near-IR probe that is spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. Cy7 Picolyl Azide is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy7 dye is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

    Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® and DyLight® are registered trademark of Thermo Fisher Scientific. IRDye® is a registered trademark of Li-Cor, Inc

    Cy7

    1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
    2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
    3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
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