Cy7 NHS Ester
Cy7 NHS Ester is a far-red (near-infrared) emitting dye with a peak excitation at 753 nm and maximum emission at 776 nm in PBS. Cy7 is spectrally almost identical to other near-IR dyes, including DyLight 750™, IRDye™ 750 dye and Alexa Fluor™ 750, and is ideal for multi-color applications. The fluorescent quantum yield and stability of Cy7 dye are remarkably improved by partially rigidizing two of the methine groups into a ring system.
DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. IRDye® is a trademark of LI-COR Biotechnology.
Cy7 NHS Ester is a water-soluble, bright, near-IR fluorescent dye with a peak excitation at 753 nm and maximum emission at 776 nm in PBS. It is spectrally similar to other 750 nm dyes (DyLight® 750, IRDye® 750 dye and Alexa Fluor® 750) used for flow cytometry, microscopy and other applications.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with Cy7 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. Cy7 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.
DyLight® Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Cy®Dye is a trademark of GE Healthcare. IRDye® is a trademark of LI-COR Biotechnology.