Cy7 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1047-1
1 mg
$115.00
In stock
1047-5
5 mg
$435.00
In stock
1047-25
25 mg
$1,295.00
In stock
1047-100
100 mg
$3,195.00
In stock

    Cy7 DBCO is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. The Cy7 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy7 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

    Abs/Em Maxima
    753/775 nm
    Extinction Coefficient
    255,000
    Spectrally Similar Dyes
    Alexa Fluor® 750, RDye® 750, CF® 750 Dye, DyLight® 750
    Molecular weight
    1259.53 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Dark green solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy7 DBCO is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. The Cy7 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy7 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

    Cy7 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy7 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    Cy7 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

    Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® and DyLight® are registered trademark of Thermo Fisher Scientific. IRDye® is a registered trademark of Li-Cor, Inc

    Cy7

    1. Van Der Wal, S., et al. (2018). Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches. Chembiochem., 19(16), 1758-65. [PubMed]
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