The Cy7 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Cy7 is a far-red (near-infrared) emitting dye with a peak excitation at 753 nm and maximum emission at 776 nm in PBS. Cy7 is spectrally almost identical to other near-IR dyes, including of Alexa Fluor® 750, DyLigh®t 750, or IRDye™ 750.
|Spectrally similar dyes||Alexa Fluor® 750,RDye® 750RD, CF™ 750 Dye, DyLight™ 750|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|
|Cy7 Picolyl Azide||A near-IR fluorescent probe with superior kinetics in copper-catalyzed click reactions.|
|Click Chemistry Protein Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|