Terazine-activated Cy5 dye reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
|Abs/Em Maxima||649/670 nm|
|Flow cytometry laser line||633 or 635 nm|
|Microscopy laser line||633 or 635 nm|
|Spectrally similar dyes||Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|