Cy5 Picolyl Azide
Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concentration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
Learn more about picolyl azide reagents.
|Abs/Em Maxima||647/663 nm|
|Flow cytometry laser line||633 or 635 nm|
|Microscopy laser line||633 or 635 nm|
|Spectrally similar dyes||Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|
1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem., 25(4): 698-706.
2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,. 51(24): 5852–5856.
3. Gaebler, A., et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res.. DOI: 10.1194/jlr.D070565.
|EZ-Click Plus MB 647 Labeling Kit||A labeling kit that provides increased sensitivity and faster reaction times compared to labeling kits based on standard azides.|
|Click Chemistry Protein Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|
|BTTAA||A newest generation, water-soluble accelerating ligand for CuAAC.|