Cy5 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1177-1
1 mg
$179.00
In stock
1177-5
5 mg
$545.00
In stock
1177-25
25 mg
$1,590.00
In stock

    Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Learn more about azide plus reagents.
    Abs/Em Maxima
    647/663 nm
    Extinction Coefficient
    250,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF® 647, DyLight® 649
    Molecular weight
    953.15 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    Cy5 Picolyl Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Cy5 dye is structurally similar, and spectrally is almost identical to Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

    2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

    3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

    4. Knutson, S.D.,et al. (2020). Chemical Profiling of A‐to‐I RNA Editing Using a Click‐Compatible Phenylacrylamide Chem. Eur.J., 26, 9874-78. [PubMed]