Water-soluble Cy®5 Maleimide is the most commonly used sulfhydryl-reactive fluorescent labeling reagents for preparing far-red-fluorescent bioconjugates. Cy5® conjugates are very bright, photostable, pH insensitive and produce minimal autofluorescence of biological specimens in this region of the spectrum. Water-soluble Cy5® dye can be used in place of Alexa Fluor 647®, DyLight 649® and other dyes excitable at a wavelength of 633 or 647 nm.
This sulfonated dye is also known as sulfo-Cyanine5 Maleimide. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||649/670 nm|
|Flow cytometry laser line||633 or 635 nm|
|Microscopy laser line||633 or 635 nm|
|Spectrally similar dyes||Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|
Protein Labeling Calculator
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Volume of DMSO required to make a stock solution (µL)
Volume of protein to be labeled (µL)
Volume of biotin stock solution to add (µL)
Percent DMSO in Reaction Mixture (%)
Caution - Volume of DSMO solvent added to protein must always be <5% total volume of reaction