Cy5 DBCO is a water-soluble, pH-insensitive from pH 4 to pH 10 bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AF™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
Cy5 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Cy5 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
|Abs/Em Maxima||649/670 nm|
|Flow cytometry laser line||633 or 635 nm|
|Microscopy laser line||633 or 635 nm|
|Spectrally similar dyes||Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|