Cy5 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
A130-1
1 mg
$115.00
In stock
A130-5
5 mg
$395.00
In stock
A130-25
25 mg
$1,095.00
In stock
A130-100
100 mg
$2,850.00
In stock

Cy5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Abs/Em Maxima
649/671 nm
Extinction Coefficient
250,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar Dyes
Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
Molecular weight
1009.22
CAS
1564286-24-3
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Cy5 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. Cy5 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Cy5 DBCO is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Cy5 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

This is sulfonated dye is also known as sulfo-Cyanine5.

Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

Cy5

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