Cy5 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
A130-1
1 mg
$115.00
In stock
A130-5
5 mg
$395.00
In stock
A130-25
25 mg
$1,095.00
In stock
A130-100
100 mg
$2,850.00
In stock

    Cy5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Abs/Em Maxima
    649/671 nm
    Extinction Coefficient
    250,000
    Flow Cytometry Laser Line
    633 or 635 nm
    Microscopy Laser Line
    633 or 635 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
    Molecular weight
    1009.22
    CAS
    1564286-24-3
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy5

    1. Yong, K.W., et al. (2020). Engineering the Orientation, Density, and Flexibility of Single-Domain Antibodies on Nanoparticles To Improve Cell Targeting. ACS Appl. Mater. Interfaces, 12(5), 5593-600. [PubMed]
    2. Song, S., et al. (2020). In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjugate Chem., 31(5), 1562-74. [PubMed]
    3. Valentini, T.V., et al. (2020). Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota. Nat Commun., 11, 2287-74. [PMC]
    Related Products
    AFDye 488 DBCO

    Excitation maximum: 494 nm
    Emission maximum: 517 nm

    AFDye 647 DBCO

    Excitation maximum: 648 nm
    Emission maximum: 671 nm

    AFDye 594 DBCO

    Excitation maximum: 590 nm
    Emission maximum: 617 nm