Cy5 DBCO NHS Ester

Unit Size
Price (USD)
1 mg
In stock
5 mg
In stock

    Cy5 DBCO NHS Ester is a bright, far red fluorescent dual-label click chemistry probe that allows for simultaneous attachment of DBCO moiety and far red fluorescent Cy5 label. Conjugates prepared with Cy5 DBCO NHS Ester reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    Cy5 dye is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Abs/Em Maxima
    649/671 nm
    Extinction Coefficient
    Flow Cytometry Laser Line
    633 or 635 nm
    Microscopy Laser Line
    633 or 635 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
    Molecular weight
    Water, DMSO, DMF
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature


    1. Bazrafshan, A. et al. (2021). DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second. ACS Publications, Online ahead of print. [PubMed]
    2. Wiener, J., et al. (2020). Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics. Sci Rep., 10 (1), 1457. [PubMed]
    3. Yong, K.W., et al. (2020). Engineering the Orientation, Density, and Flexibility of Single-Domain Antibodies on Nanoparticles To Improve Cell Targeting. ACS Appl. Mater. Interfaces, 12(5), 5593-600. [PubMed]
    4. Song, S., et al. (2020). In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjugate Chem., 31(5), 1562-74. [PubMed]
    5. Valentini, T.V., et al. (2020). Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota. Nat Commun., 11, 2287-74. [PMC]
    6. Kim, S. H., et al. (2014). Cell labeling and tracking method without distorted signals by phagocytosis of macrophages . Theranostics, 4 (4), 420-31. [PubMed]
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