Cy5 Biotin

Catalog#
Unit Size
Price (USD)
Availability
Qty
1049-1
1 mg
$149.00
In stock
1049-5
5 mg
$495.00
In stock

    Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

    Abs/Em Maxima
    649/671 nm
    Extinction Coefficient
    250,000
    Flow Cytometry Laser Line
    633 or 635 nm
    Microscopy Laser Line
    633 or 635 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
    Molecular weight
    1136.39
    CAS
    N/A
    Solubility
    Water, DMSO, DMF, MeOH
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy5

    1. Schendel, L. C., et al. (2020). Single-Molecule Manipulation in Zero-Mode Waveguides. Small., 16 (13), e1906740. [PubMed]
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