Cy5 Biotin
Catalog#
Unit Size
Price (USD)
Availability
Qty
1049-1
1 mg
$165.00
In stock
1049-5
5 mg
$545.00
In stock
Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Abs/Em Maxima
649/671 nm
Extinction Coefficient
250,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar Dyes
Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
Molecular weight
1136.39
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature
- Schendel, L. C., et al. (2020). Single-Molecule Manipulation in Zero-Mode Waveguides. Small., 16 (13), e1906740. [PubMed]
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