Cy5 Biotin Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1232-1
1 mg
$195.00
In stock
1232-5
5 mg
$495.00
In stock
1232-25
25 mg
$2,079.00
In stock

    Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label Cy5 Biotin Azide probe can be readily incorporated into alkyne-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence.

    Molecular weight
    1494.81
    Chemical composition
    C57H79N11O14S
    CAS
    N/A
    Solubility
    DMSO, DMF
    Appearance
    Dark blue solid
    Storage conditions
    -20°C.
    Shipping conditions
    Ambient temperature

    Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label TAMRA Biotin Azide probe can be readily incorporated into alkyne-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence.

    Another very important advantage of dual label probes over regular biotin probes is built-in control. Each step of enrichment process can easily followed either by UV-Vis (650 nm) or by more sensitive fluorescence spectroscopy. After elution form streptavidin beads target proteins containing TAMRA label can be easily distinguished from non-specifically bound proteins and endogenously biotinlytated proteins.

    This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.

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