Cy5 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
AZ118-1
1 mg
$139.00
In stock
AZ118-5
5 mg
$379.00
In stock
AZ118-25
25 mg
$850.00
In stock
AZ118-100
100 mg
$2,650.00
In stock

    The far-red fluorescent Cy5 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Abs/Em Maxima
    649/671 nm
    Extinction Coefficient
    250,000
    Flow Cytometry Laser Line
    633 or 635 nm
    Microscopy Laser Line
    633 or 635 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
    Molecular weight
    834.01 (protonated)
    CAS
    n/a
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy5 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

    Cy5 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    This is sulfonated dye is also known as sulfo-Cyanine5.

    Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    Cy5

    1. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]
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