The NIR-fluorescent Cy5 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Cy5 is a water-soluble, bright fluorescent label that can be used in place of Alexa Fluor 647®, DyLight 649® and other dyes excitable at a wavelength of 633 or 647 nm.
For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.
This sulfonated dye is also known as sulfo-Cyanine5 Azide. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||647/663 nm|
|Flow cytometry laser line||633 or 635 nm|
|Microscopy laser line||633 or 635 nm|
|Spectrally similar dyes||Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|
|Cy5 Picolyl Azide||A near-IR fluorescent probe with superior kinetics in copper-catalyzed click reactions.|
|Click Chemistry Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|