Cy5 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
TA116-1
1 mg
$139.00
In stock
TA116-5
5 mg
$410.00
In stock
TA116-25
25 mg
$895.00
In stock
TA116-100
100 mg
$2,650.00
In stock

    The far-red fluorescent Cy5 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Abs/Em Maxima
    649/671 nm
    Extinction Coefficient
    250,000
    Flow Cytometry Laser Line
    633 or 635 nm
    Microscopy Laser Line
    633 or 635 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF™ 647 Dye, DyLight™649
    Molecular weight
    787.96 (protonated)
    CAS
    n/a
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy5 Alkyne can be reacted with azides (N3) via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

    Cy5 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    This is sulfonated dye is also known as sulfo-Cyanine5.

    Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    Cy5

    1. Banerjee, C., et al. (2022). Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET. J Vis Exp., 186. [PubMed]
    2. Schamber, M. R., et al. (2022). Conformational fingerprinting of allosteric modulators in metabotropic glutamate receptor 2. Elife, 11:e78982. [PubMed]
    3. Schamber, M. R., et al. (2022). Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning. Nat Commun., 13 (1), 2194. [PubMed]
    4. Zhang, C., et al. (2016). In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy. Proc Natl Acad Sci U S A., 113 (19), 5173-8. [PubMed]
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