Cy5 Alkyne
The far-red fluorescent Cy5 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
Cy5 Alkyne can be reacted with azides (N3) via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
Cy5 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
This is sulfonated dye is also known as sulfo-Cyanine5.
Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
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Excitation maximum: 590 nm
Emission maximum: 617 nm
Excitation maximum: 648 nm
Emission maximum: 671 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm