Cy5.5 NHS Ester
Cy5.5 NHS Ester is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680. Cy5.5 NHS Ester is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents.
This sulfonated dye is also known as sulfo-Cyanine5.5 NHS ester.
DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. IRDye® is a trademark of LI-COR Biotechnology.
Cy5.5 NHS Ester (also sold under sulfo-Cyanine5.5 NHS Ester name) is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. Cy5.5 NHS Ester is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents. Cy5.5 conjugates are pH insensitive from pH 4 to pH 10 and produce minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
The NHS ester (succinimidyl ester) of Cy5.5 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
Cy5.5 spectrally is almost identical to Alexa Fluor® 680, CF® 680 Dye, IRDye® 680 or any other Cyanine5.5 based fluorescent dyes, and can be used as a less expensive alternative to these dyes.
DyLight® Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Cy®Dye is a trademark of GE Healthcare. IRDye® is a trademark of LI-COR Biotechnology.
