Cy5.5 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1046-1
1 mg
$105.00
In stock
1046-5
5 mg
$395.00
In stock
1046-25
25 mg
$1,195.00
In stock
1046-100
100 mg
$2,950.00
In stock

Cy5.5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Cy5.5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Abs/Em Maxima
678/694 nm
Extinction Coefficient
190,000
Spectrally Similar Dyes
Alexa Fluor® 680, IRDye® 680RD, DyLight® 680
Molecular weight
1175.37
CAS
1857352-95-4
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Cy5.5 DBCO is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. The Cy5.5 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy5.5 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

Cy5.5 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5.5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Cy5.5 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® and DyLight® are registered trademark of Thermo Fisher Scientific. IRDye® is a registered trademark of Li-Cor, Inc

Cy5.5

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