Cy5.5 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5.5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Cy5.5 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
|Spectrally similar dyes||Alexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye, DyLight™ 680|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|