The NIR-fluorescent Cy5.5 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Cy5.5 is a water-soluble, pH-insensitive, bright fluorescent probe that can be used in place of Alexa Fluor® 680, DyLigh®t 680, or IRDye™ 680 and other dyes excitable at a wavelength at around 680 nm.
This sulfonated dye is also known as sulfo-Cyanine5.5 Azide. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||678/694 nm|
|Spectrally similar dyes||Alexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye, DyLight™ 680|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|
|Cy5.5 Picolyl Azide||A near-IR fluorescent probe with superior kinetics in copper-catalyzed click reactions.|
|Click Chemistry Protein Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|