Cy3 Tyramide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1545-1
1 mg
$149.00
In stock
1545-5
5 mg
$585.00
In stock

    Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

    Cy3 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

    Abs/Em Maxima
    555/572 nm
    Extinction Coefficient
    155,000
    Flow Cytometry Laser Line
    532 nm or 555 nm
    Microscopy Laser Line
    532 nm or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
    Molecular weight
    735.91 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye555

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