Cy3 NHS Ester
Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
The NHS ester (also known as succinimidyl esters) is the most popular amine-reactive variation of Cy®3 dye used for labeling proteins, antibodies, peptides, amine-modified oligonucleotides, and other amine-containing molecules with this dye.
This sulfonated dye is also known as sulfo-Cyanine3 NHS ester. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||550/570 nm|
|Flow cytometry laser line||532 or 555 nm|
|Microscopy laser line||532 or 555 nm|
|Spectrally similar dyes||TAMRA, Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|