Cy3 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1075-1
1 mg
$105.00
In stock
1075-5
5 mg
$295.00
In stock
1075-25
25 mg
$695.00
In stock
1075-100
100 mg
$1,950.00
In stock

Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

This sulfonated dye is also known as sulfo-Cyanine3 NHS ester.

Abs/Em Maxima
553/569 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes
Alexa Fluor® 555, CF® 555, DyLight® 549
Molecular weight
713.82 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Cy3 NHS ester is not recommended for labeling proteins at high molar ration due significant self-quenching, and only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AFDye 555 NHS Ester, which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Cy3 is spectrally is almost identical to Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

Cy3

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