Cy3 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1075-1
1 mg
$95.00
In stock
1075-5
5 mg
$295.00
In stock
1075-25
25 mg
$700.00
In stock
1075-100
100 mg
$1,550.00
In stock

    Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

    This sulfonated dye is also known as sulfo-Cyanine3 NHS ester.

    Abs/Em Maxima
    553/569 nm
    Extinction Coefficient
    155,000
    Flow Cytometry Laser Line
    532 nm or 555 nm
    Microscopy Laser Line
    532 nm or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, CF® 555, DyLight® 549
    Molecular weight
    713.82 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

    The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Cy3 NHS ester is not recommended for labeling proteins at high molar ration due significant self-quenching, and only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AFDye 555 NHS Ester, which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

    Cy3 is spectrally is almost identical to Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

    Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

    Cy3

    Related Products
    AFDye 594 DBCO

    Excitation maximum: 590 nm
    Emission maximum: 617 nm

    AFDye 647 DBCO

    Excitation maximum: 648 nm
    Emission maximum: 671 nm