Cy3 Methyltetrazine

Catalog#
Unit Size
Price (USD)
Availability
Qty
1018-1
1 mg
$149.00
In stock
1018-5
5 mg
$435.00
In stock
1018-25
25 mg
$1,650.00
In stock

    Methyltetrazine-activated Cy3 probe that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1 -1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

    Abs/Em Maxima
    553/569 nm
    Extinction Coefficient
    150,000
    Flow Cytometry Laser Line
    532 or 555 nm
    Microscopy Laser Line
    532 or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549
    Molecular weight
    908.07
    CAS
    1676067-48-3
    Solubility
    Water, DMSO, DMF, MeOH
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Methyltetrazine-activated Cy3 probe that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1 -1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date. described to date.

    Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

    Cy®Dye is a trademark of GE Healthcare

    Cy3

    1. Marquard, A.N., et al. (2020). Expanding the Scope of Antibody Rebridging with New Pyridazinedione-TCO Constructs. Bioconjug Chem., 31(6), 1616-23. [PubMed]
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