Methyltetrazine-activated Cy3 dye that reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 -1 s-1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
Cy®Dye is a trademark of GE Healthcare
|Abs/Em Maxima||550/570 nm|
|Flow cytometry laser line||532 555 nm|
|Microscopy laser line||532 or 555 nm|
|Spectrally similar dyes||Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|